Hiki ke hoʻohana ʻia nā pā palaha no nā ʻano cell āpau, akā inā liʻiliʻi ka helu o nā cell, e like me ka cloning, hoʻohana ʻia kahi pā 96-well.
Eia hou, hoʻohana mau ʻia nā pā palaha no ka MTT a me nā hoʻokolohua ʻē aʻe, no ka pā a me ka hoʻopaneʻe ʻia.
Hoʻohana pinepine ʻia nā pā U a i ʻole V no nā koi kikoʻī.No ka laʻana, i ka immunology, ke hui ʻia nā lymphocytes ʻelua ʻokoʻa, pono lākou e launa pū kekahi me kekahi no ka hoʻoulu ʻana.No laila, koi pinepine ʻia nā pā U no ka mea e hōʻiliʻili nā cell i kahi wahi liʻiliʻi ma muli o ka umekaumaha.Hoʻohana liʻiliʻi ʻia nā pā V no nā hoʻokolohua pepehi cell e hoʻopili kokoke i nā cell target, akā hiki ke hoʻololi ʻia e nā pā U (ma hope o ka hoʻohui ʻana o nā cell, centrifugation me ka wikiwiki haʻahaʻa).
No ka moʻomeheu cell, hoʻohana mau ʻia nā papa pālahalaha, me ka nānā kūikawā i ka mea.
Hoʻohana mau ʻia nā poʻe lalo no ka nānā ʻana, ka hopena kemika a i ʻole ka waiho ʻana i nā laʻana.No ka mea, ʻoi aku ka maikaʻi o ka lalo poepoe i ka hoʻomaʻemaʻe ʻana i ka wai, ma mua o ka lalo palahalaha.Eia nō naʻe, inā e ana ʻoe i nā waiwai absorbance, pono ʻoe e kūʻai mau i kahi papa pālahalaha.
ʻO ka hapa nui o nā papa moʻomeheu cell he papa pālahalaha no ka nānā ʻana i nā microscopic maʻalahi, kahi ʻāpana o lalo, kahi pae moʻomeheu like ʻole a no ka hoʻāʻo MTT.
Hoʻohana nui ʻia nā papa pōʻai lalo no nā hoʻokolohua isotope doping kahi e pono ai e hōʻiliʻili ʻia nā cell me kahi mea hōʻiliʻili cell, e like me 'mixed lymphocyte culture'.